5ZCD

Crystal structure of Alpha-glucosidase in complex with maltotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	55.628, 84.745, 128.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.504 - 1.707
R-factor	0.1792
Rwork	0.178
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ze0
RMSD bond length	0.006
RMSD bond angle	1.041
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.700	1.700
Rmerge	0.107	0.675
Rmeas	0.117	0.743
Number of reflections	66627	10452
<I/σ(I)>	11.1	2
Completeness [%]	99.6	97.8
Redundancy	6.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG 3350, Calcium Chloride