5ZC4

Crystal Structure of RNF13 RING domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	MARRESEARCH
Wavelength(s)	0.97947
Spacegroup name	P 62
Unit cell lengths	90.338, 90.338, 45.445
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.619 - 1.906
R-factor	0.1671
Rwork	0.166
R-free	0.18580
Structure solution method	SIRAS
RMSD bond length	0.015
RMSD bond angle	1.431
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.170	1.950
High resolution limit [Å]	1.906	1.906
Rmerge	0.051	0.813
Rpim	0.018	0.298
Number of reflections	16628	1071
<I/σ(I)>	24.6	2.3
Completeness [%]	99.4	100
Redundancy	8.8	7.9
CC(1/2)	0.999	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100% Tacsimate