5ZBK

Crystal structure of type-I LOG from Pseudomonas aeruginosa PAO1 in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	64.844, 97.524, 71.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.000 - 2.300
R-factor	0.1847
Rwork	0.182
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5its
RMSD bond length	0.014
RMSD bond angle	1.780
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.123	0.349
Rpim	0.063	0.162
Number of reflections	10160	493
<I/σ(I)>	12.8
Completeness [%]	97.3
Redundancy	4.3
CC(1/2)	0.912	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	ammonium phosphate dibasic, citrate pH 5.5, sodium chloride