5ZBJ

Crystal structure of type-I LOG from Pseudomonas aeruginosa PAO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	64.931, 97.917, 71.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.110 - 1.890
R-factor	0.1541
Rwork	0.152
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5its
RMSD bond length	0.018
RMSD bond angle	1.839
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.110	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.072	0.347
Rpim	0.036	0.158
Number of reflections	18128	845
<I/σ(I)>	4.5
Completeness [%]	98.1
Redundancy	5
CC(1/2)	0.804	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	ammonium phosphate dibasic, imidazole pH 8.0