5ZBB

Crystal structure of Rtt109-Asf1-H3-H4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9788
Spacegroup name	P 65 2 2
Unit cell lengths	112.251, 112.251, 333.057
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.921 - 3.600
R-factor	0.2179
Rwork	0.215
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zb9 2hue
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.730
High resolution limit [Å]	3.600	7.750	3.600
Rmerge	0.249	0.055	0.831
Rmeas	0.256	0.057	0.853
Rpim	0.058	0.013	0.195
Number of reflections	15314	1728	1475
<I/σ(I)>	3.7
Completeness [%]	99.9	99.5	99.9
Redundancy	18.9	18.1	19.1
CC(1/2)		0.999	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	100mM sodium citrate, pH 5.0, 22% PEG 1500, 400mM sodium iodide