5ZB7
CTX-M-64 apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.123, 106.898, 47.521 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 46.830 - 1.630 |
| R-factor | 0.19509 |
| Rwork | 0.193 |
| R-free | 0.22775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5twd |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.450 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Number of reflections | 78497 | |
| <I/σ(I)> | 12.262 | |
| Completeness [%] | 98.5 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M HEPES pH 7.0, 25% PEG3350 |
