5ZB5
The structural basis of histone chaperoneVps75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2014-07-23 |
| Detector | RIGAKU |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 66.410, 66.410, 99.417 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.959 - 2.299 |
| R-factor | 0.1835 |
| Rwork | 0.180 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yps |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.299 | 2.299 |
| Number of reflections | 19190 | |
| <I/σ(I)> | 37.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Bis-Tris Propane (pH =7.0) and 20M Ammonium citrate tribasic |
