5ZB2

Crystal structure of Rad7 and Elc1 complex in yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 2
Unit cell lengths	110.921, 67.764, 69.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.353 - 2.300
R-factor	0.197077370165
Rwork	0.195
R-free	0.22407
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.530
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXCD
Refinement software	phenix.refine (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.110	0.057	0.682
Rmeas	0.116	0.061	0.713
Rpim	0.035	0.020	0.203
Number of reflections	24237	1357	1186
<I/σ(I)>	6.3
Completeness [%]	97.5	98.7	96.3
Redundancy	10.8	10.3	11.7
CC(1/2)		0.996	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1 M KCl, 0.1 M sodium Citrate tribasic dihydrate, 22% PEG 3350, 0.1 M Yttrium(III) cloride hexahydrate