5ZAS
Crystal structure of 5-formylcytosine containing decamer dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 180 |
| Detector technology | PIXEL |
| Collection date | 2016-06-13 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9676 |
| Spacegroup name | P 61 |
| Unit cell lengths | 54.548, 54.548, 45.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.240 - 1.560 |
| R-factor | 0.14736 |
| Rwork | 0.147 |
| R-free | 0.16210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 11176 | |
| <I/σ(I)> | 37.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50mM cacodylate pH 6.0, 20mM magnesium acetate, 0.5mM spermine, 100mM NaCl, 25% MPD |
