5ZA0
A cryo-protectant induces the conformational change of glyceraldehyde-3-phosphate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 121.295, 121.295, 156.056 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.880 - 2.000 |
R-factor | 0.18485 |
Rwork | 0.184 |
R-free | 0.20474 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s7c |
RMSD bond length | 0.010 |
RMSD bond angle | 1.284 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.880 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 39522 | |
<I/σ(I)> | 21.8 | |
Completeness [%] | 99.6 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277.15 | Ammonium sulfate, MES |