5Z9O
The crystal structure of Cyclopropane-fatty-acyl-phospholipid synthase from Lactobacillus acidophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-11 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 120.982, 120.982, 163.487 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.106 - 2.700 |
| R-factor | 0.17696 |
| Rwork | 0.175 |
| R-free | 0.21258 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l1e |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.814 |
| Data reduction software | PDBSET |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.210 | 2.773 |
| High resolution limit [Å] | 2.700 | 2.703 |
| Number of reflections | 37057 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 1.5 M ammonium sulfate, 0.1 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dehydrate |
