5Z9G
Crystal structure of KAI2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 93.1 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.599, 55.785, 53.034 |
| Unit cell angles | 90.00, 116.07, 90.00 |
Refinement procedure
| Resolution | 47.640 - 1.490 |
| R-factor | 0.13854 |
| Rwork | 0.136 |
| R-free | 0.17662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wom |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.929 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 43387 | |
| <I/σ(I)> | 54.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 290 | 1M Na-citrate, 100 mM HEPES (pH 7.5), 5% glycerol |
