5Z8Y

Crystal structure of human LRRTM2 in complex with Neurexin 1beta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	99.184, 99.858, 109.633
Unit cell angles	91.30, 91.20, 116.47

Refinement procedure

Resolution	49.815 - 3.400
R-factor	0.2104
Rwork	0.209
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z8x 3b3q
RMSD bond length	0.004
RMSD bond angle	0.890
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.400	3.400
Number of reflections	49781
<I/σ(I)>	7.2
Completeness [%]	96.3	93.1
Redundancy	3	2.5
CC(1/2)		0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	15% PEG 3350, 0.1 M sodium/potassium phosphate