5Z8S
Human Mitochondrial ferritin mutant - C102A/C130A/E27C/E61C/E62C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 182.736, 182.736, 182.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.923 - 1.973 |
R-factor | 0.1676 |
Rwork | 0.166 |
R-free | 0.19950 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.799 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.923 | 2.044 |
High resolution limit [Å] | 1.973 | 1.973 |
Number of reflections | 18997 | |
<I/σ(I)> | 5.4 | |
Completeness [%] | 100.0 | |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 0.1M Tris-HCl, 3.4M 1,6-Hexanediol |