5Z8B

Truncated N-acetylglucosaminyl transferase KfiA from E. coli K5 strain apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	38.409, 70.862, 147.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.710 - 2.910
R-factor	0.18227
Rwork	0.178
R-free	0.26517
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.588
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.710	3.080
High resolution limit [Å]	2.910	2.910
Number of reflections	16958
<I/σ(I)>	8.9
Completeness [%]	99.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	PEG 3350