5Z83

Crystal structure of GenB1 from Micromonospora echinospora in complex with PLP (internal aldimine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-15
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	77.140, 88.012, 116.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.798 - 1.700
R-factor	0.1591
Rwork	0.158
R-free	0.19230
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.190
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2474)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.055	0.241
Rpim	0.017	0.123
Number of reflections	84747	3524
<I/σ(I)>	34
Completeness [%]	97.2
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	magnesium formate, PEG3350