5Z7Z

Crystal structure of Striga hermonthica Dwarf14 (ShD14)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2015-10-31
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	44.662, 70.316, 84.684
Unit cell angles	90.00, 94.03, 90.00

Refinement procedure

Resolution	38.313 - 1.978
R-factor	0.2103
Rwork	0.208
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vxk
RMSD bond length	0.004
RMSD bond angle	0.648
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.978	1.978
Rmeas	0.065	0.582
Number of reflections	36505	2707
<I/σ(I)>	17.6	3
Completeness [%]	99.6	99.8
Redundancy	3.8	3.8
CC(1/2)	0.999	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	293.15	100mM CAPS (pH 10.0), 200mM NaCl, 20% (w/v) PEG 8000