5Z7G
Crystal structure of TAX1BP1 SKICH region in complex with NAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-05 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 63 |
| Unit cell lengths | 118.487, 118.487, 64.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.622 - 2.301 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z7a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.853 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 22896 | |
| <I/σ(I)> | 36.33 | |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | PEG 8000, 0.1 M HEPES |
