5Z7F

Horse Heart Myoglobin Mutant -V68A/I107Y-Sulfide Derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97845
Spacegroup name	P 1 21 1
Unit cell lengths	35.003, 28.516, 62.696
Unit cell angles	90.00, 105.61, 90.00

Refinement procedure

Resolution	60.380 - 1.900
R-factor	0.16674
Rwork	0.164
R-free	0.22740
RMSD bond length	0.017
RMSD bond angle	1.845
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.380	1.949
High resolution limit [Å]	1.900	1.900
Number of reflections	8914
<I/σ(I)>	15
Completeness [%]	97.0
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.5-3.0M ammonium sulfate, 0.1M Tris