5Z7A
Crystal structure of NDP52 SKICH region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-10 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 31 |
| Unit cell lengths | 46.502, 46.502, 99.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.333 - 2.380 |
| R-factor | 0.2238 |
| Rwork | 0.222 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vvv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.589 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 9783 | |
| <I/σ(I)> | 12.49 | |
| Completeness [%] | 90.3 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 289 | PEG 3350, 0.2 M potassium sulfate |
