5Z71

Crystal structure of the Homo Sapiens cytoplasmic ribosomal decoding site in complex with G418 (P21212 form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	30.924, 90.106, 46.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.053 - 2.500
R-factor	0.2731
Rwork	0.270
R-free	0.30510
RMSD bond length	0.015
RMSD bond angle	1.487
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.053	45.053	2.560
High resolution limit [Å]	2.500	11.170	2.500
Rmerge	0.029	0.022	0.313
Rmeas	0.034	0.026	0.364
Number of reflections	8669	66	648
<I/σ(I)>	25.53	46.26	3.61
Completeness [%]	98.9	66.7	97.9
Redundancy	3.827	3.424	3.73
CC(1/2)	0.999	0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MPD, Sodium chloride, Sprimine 4HCl, Sodium cacodylate (pH 5.0)