5Z6Z

Crystal structure of human DUX4 homeodomains bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	64.879, 64.879, 152.075
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.838 - 2.301
R-factor	0.2135
Rwork	0.211
R-free	0.25060
RMSD bond length	0.004
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.200	2.310
High resolution limit [Å]	2.250	2.250
Number of reflections	18746
<I/σ(I)>	23.43
Completeness [%]	99.8
Redundancy	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 3350