5Z6T
Crystal structure of D-xylose reductase from Scheffersomyces stipitis in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 97.654, 97.654, 160.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.370 - 2.000 |
| R-factor | 0.1799 |
| Rwork | 0.177 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z9a |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.380 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 49336 | 2503 |
| <I/σ(I)> | 26.3 | |
| Completeness [%] | 98.6 | |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Ammonium citrate tribasic |
