5Z6O

Crystal structure of Penicillium cyclopium protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1995-04-01
Detector	UCSD MARK II
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	59.223, 61.642, 70.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.721 - 1.700
R-factor	0.1441
Rwork	0.142
R-free	0.18090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2prk
RMSD bond length	0.011
RMSD bond angle	1.073
Data reduction software	UCSD-system
Data scaling software	Aimless
Phasing software	X-PLOR
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.088	0.362
Number of reflections	33985	1580
<I/σ(I)>	10.6	2.8
Completeness [%]	97.5	92.7
Redundancy	5.3	3.3
CC(1/2)	0.996	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15% PEG 4000