5Z6D

Crystal structure of Abundant Perithecial Protein (APP) from Neurospora crassa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2010-06-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	46.319, 40.168, 103.542
Unit cell angles	90.00, 96.81, 90.00

Refinement procedure

Resolution	23.031 - 1.600
R-factor	0.1859
Rwork	0.184
R-free	0.22890
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	1.039
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.053	0.245
Number of reflections	45708	3831
<I/σ(I)>	29.2	6
Completeness [%]	91.1	77.4
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20-25% v/v PEG MME 550, 10-30mM CaCl2, 100mM Bis-Tris, pH 5.5-6.5