5Z69
Structure of the recombination mediator protein RecF-ATPrS in RecFOR pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.005 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.727, 48.107, 116.855 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 19.835 - 2.102 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.22070 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 913873 | |
| <I/σ(I)> | 31.2 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 300 mM NaCl, 100 mM Tris-Hcl, pH 7.0 |
