5Z67

Structure of the recombination mediator protein RecF in RecFOR pathway

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.005
Spacegroup name	C 2 2 21
Unit cell lengths	49.839, 95.780, 167.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.992 - 2.200
R-factor	0.1792
Rwork	0.175
R-free	0.22310
RMSD bond length	0.010
RMSD bond angle	1.262
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.200	2.200
<I/σ(I)>	60.7
Completeness [%]	99.0
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	293	28% (w/v) PEG 3350, 300 mM NaCl, 100 mM Tris-Hcl, pH 9.0