5Z5P
Crystal structure of ConA-R3M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9716 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.742, 61.945, 115.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.740 - 1.400 |
| R-factor | 0.19492 |
| Rwork | 0.193 |
| R-free | 0.24149 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 84005 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 300 | 20% polyethylene glycol 6000, 20 mM HEPES, 100 mM NaCl, 5 mM CaCl2, 5 mM MnCl2 pH 7.2 |
