5Z5B
Crystal structure of Tk-PTP in the G95A mutant form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2017-09-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 154.796, 154.796, 75.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.400 - 2.300 |
| R-factor | 0.2216 |
| Rwork | 0.219 |
| R-free | 0.26459 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z5a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.400 | 2.340 |
| High resolution limit [Å] | 2.298 | 2.298 |
| Number of reflections | 28515 | |
| <I/σ(I)> | 27 | |
| Completeness [%] | 95.2 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris (pH 6.25) and 0.8M magnesium formate dehydrate |
