5Z4W

Crystal structure of signalling protein from buffalo (SPB-40) with an altered conformation of Trp78 at 1.79 A resolution

Replaces:  5WSM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-02
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	61.068, 66.735, 106.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.490 - 1.790
R-factor	0.152
Rwork	0.150
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q7n
RMSD bond length	0.030
RMSD bond angle	2.529
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.500	1.840
High resolution limit [Å]	1.790	1.790
Number of reflections	40939
<I/σ(I)>	2.1
Completeness [%]	99.6
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	35mM Tris-HCl, 25mM NaCl, 20% ethanol pH 8.0