5Z4A

Structure of Tailor in complex with AGU RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	C 2 2 21
Unit cell lengths	61.717, 84.944, 148.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.930 - 1.637
R-factor	0.1587
Rwork	0.157
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nkt
RMSD bond length	0.009
RMSD bond angle	0.979
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.890
High resolution limit [Å]	1.637	5.050	1.860
Rmerge	0.086	0.055	0.493
Rmeas	0.089	0.057	0.515
Rpim	0.025	0.016	0.149
Number of reflections	48194	1771	1442
<I/σ(I)>	4.6
Completeness [%]	99.5	100	93.1
Redundancy	12.8	12.3	10.8
CC(1/2)		0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M Lithium chloride, 0.1M Tris pH 8.4, 15% w/v PEG 3350