5Z43
Crystal structure of prenyltransferase AmbP1 apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-09 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.1000 |
Spacegroup name | P 41 |
Unit cell lengths | 116.263, 116.263, 48.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.105 - 2.361 |
R-factor | 0.1939 |
Rwork | 0.190 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yk9 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.973 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.900 | 2.450 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.038 | 0.493 |
Number of reflections | 27028 | 2823 |
<I/σ(I)> | 18.8 | 2.3 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 3.9 | 3.9 |
CC(1/2) | 0.999 | 0.807 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM MES (pH6.5), 0.2M MgCl2, 22% PEG8000 |