5Z3F
Glycosidase E335A in complex with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-04 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 104.830, 104.830, 89.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.880 - 1.100 |
R-factor | 0.10827 |
Rwork | 0.108 |
R-free | 0.12089 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.695 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.880 | 1.160 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 200183 | |
<I/σ(I)> | 16.39 | |
Completeness [%] | 99.9 | |
Redundancy | 18.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Ammonium citrate tribasic (pH 6.8), 0.1M Sodium citrate (pH 5.5), 12% PEGmonomethyl ether 2000, 2mM isomaltose |