5Z39

Crystal structure of C terminal region of G-protein interacting protein 1 (Gip1) from Dictyostelium discoideum form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-22
Detector	RAYONIX MX225HE
Wavelength(s)	1.700
Spacegroup name	P 21 21 21
Unit cell lengths	33.468, 43.618, 101.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.085 - 2.740
R-factor	0.2265
Rwork	0.221
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z1n
RMSD bond length	0.002
RMSD bond angle	0.454
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.620	43.620	2.870
High resolution limit [Å]	2.740	9.090	2.740
Rmerge	0.122	0.044	0.611
Rmeas	0.127	0.046	0.634
Rpim	0.034	0.013	0.169
Total number of observations	57795
Number of reflections	4277	150	554
<I/σ(I)>	18.9
Completeness [%]	100.0	99	100
Redundancy	13.5	10.4	13.9
CC(1/2)	0.999	0.999	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14-20% PEG 20000, 100 mM Bicine,