5Z2M
Structure of Orp1L/Rab7 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.222, 109.626, 77.811 |
| Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
| Resolution | 25.586 - 2.142 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t91 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.002 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 38402 | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 98.2 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M HEPES pH 7.5, 4% PEG 8000 |
