5Z2I

Structure of Dictyostelium discoideum mitochondrial calcium uniporter N-ternimal domain (Se-DdMCU-NTD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	P 1 21 1
Unit cell lengths	42.455, 70.832, 60.005
Unit cell angles	90.00, 92.17, 90.00

Refinement procedure

Resolution	42.425 - 2.141
R-factor	0.1927
Rwork	0.191
R-free	0.23420
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.740
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.770	2.200
High resolution limit [Å]	2.140	2.140
Rmerge	0.099	0.823
Rmeas	0.105	0.881
Rpim	0.034	0.305
Number of reflections	19374	1416
<I/σ(I)>	13.9	2.8
Completeness [%]	98.7	88.6
Redundancy	9.4	8
CC(1/2)	0.998	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20%(v/v) PEG4000, 10%(v/v) 2-propanol, 0.01M HEPES (pH 7.5)