5Z22
Crystal Structure of Laccase from Cerrena sp. RSD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-03 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 87.409, 87.409, 148.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.12699 |
| Rwork | 0.126 |
| R-free | 0.15195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z1x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.064 | 0.630 |
| Number of reflections | 89774 | 8972 |
| <I/σ(I)> | 27.5 | 1.9 |
| Completeness [%] | 96.8 | 98.1 |
| Redundancy | 4.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 1.6M almmonium sulfate, pH8.4 |
