5Z21

The ternary structure of D-lactate dehydrogenase from Fusobacterium nucleatum with NADH and oxamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97911
Spacegroup name	P 21 21 2
Unit cell lengths	117.230, 159.740, 90.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.010 - 2.300
R-factor	0.22679
Rwork	0.225
R-free	0.25142
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j49
RMSD bond length	0.014
RMSD bond angle	1.629
Data scaling software	SCALA
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.010	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.125	0.640
Rpim	0.050	0.254
Number of reflections	75787	10924
<I/σ(I)>	13.5	3.2
Completeness [%]	100.0	100
Redundancy	7.2	7.3
CC(1/2)	0.996	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	2 mM NADH, 25 mM oxamate, 100 mM acetate buffer pH 5, 4% PEG 4000