5Z1Q

Crystal structures of the trimeric N-terminal domain of Ciliate Euplotes octocarinatus Centrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9779
Spacegroup name	P 1
Unit cell lengths	42.945, 43.045, 43.087
Unit cell angles	106.88, 106.89, 106.61

Refinement procedure

Resolution	37.653 - 2.300
R-factor	0.1859
Rwork	0.184
R-free	0.22430
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.386
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.10.1)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmeas	0.246	0.312
Number of reflections	9561
<I/σ(I)>	5.27
Completeness [%]	97.5
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	100mM NaH2PO4 (pH=4.2) and 40%(w/v) PEG600