5Z1N

Crystal structure of C terminal region of G-protein interacting protein 1 (Gip1) from Dictyostelium discoideum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-20
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	33.318, 44.143, 96.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.319 - 1.949
R-factor	0.1847
Rwork	0.182
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f4m
RMSD bond length	0.003
RMSD bond angle	0.602
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.949	5.290	1.950
Rmerge	0.090	0.045	0.353
Rmeas	0.105	0.054	0.424
Rpim	0.054	0.030	0.232
Number of reflections	10906	645	359
<I/σ(I)>	8.2
Completeness [%]	98.3	99.5	66.2
Redundancy	3.8	3.3	3.2
CC(1/2)		0.996	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14-20% PEG 20000, 100mM Bicine,