5Z1H

Crystal structure of the bacterial ribosomal decoding site in complex with 6'-fluoro sisomicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-14
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	42.905, 31.665, 46.696
Unit cell angles	90.00, 94.82, 90.00

Refinement procedure

Resolution	42.800 - 2.400
R-factor	0.2171
Rwork	0.212
R-free	0.26320
RMSD bond length	0.007
RMSD bond angle	1.008
Data scaling software	d*TREK (9.4SSI)
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.800	42.750	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.084	0.057	0.272
Rmeas	0.084
Number of reflections	5052	523	486
<I/σ(I)>	8.5	16.5	3.2
Completeness [%]	99.7	98.1	100
Redundancy	3.54	3.29	3.61

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2-Methyl-2,4-pentanediol, LiCl, spermine tetrahydrochloride, sodium cacodylate