5Z15
Crystal structure of human TLR8 in complex with CU-CPT9c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-10-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.892, 98.747, 139.476 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 131.850 - 2.900 |
| R-factor | 0.2059 |
| Rwork | 0.202 |
| R-free | 0.28922 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.591 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.850 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 41014 | |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 98.8 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4000, calcium chloride, Tris-HCl, ethylene glycol |
