5Z0B

Crystal structure of plasma-derived human serum albumin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9536
Spacegroup name	C 1 2 1
Unit cell lengths	203.576, 113.466, 86.809
Unit cell angles	90.00, 104.88, 90.00

Refinement procedure

Resolution	19.780 - 2.170
R-factor	0.1853
Rwork	0.183
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ao6
RMSD bond length	0.008
RMSD bond angle	0.914
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.780	2.290
High resolution limit [Å]	2.170	2.170
Number of reflections	96299	11416
<I/σ(I)>	10.8	2.7
Completeness [%]	96.2	78.3
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	PEG600, Ammonium sulfate, MES