5Z0A
ST0452(Y97N)-GlcNAc binding form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 90.843, 94.951, 101.906 |
Unit cell angles | 110.62, 101.47, 113.03 |
Refinement procedure
Resolution | 87.790 - 2.090 |
R-factor | 0.19481 |
Rwork | 0.193 |
R-free | 0.23154 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ggo |
RMSD bond length | 0.017 |
RMSD bond angle | 1.859 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.800 | 2.150 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.072 | 0.415 |
Number of reflections | 154041 | 7531 |
<I/σ(I)> | 22 | |
Completeness [%] | 96.9 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% (w/v) PEG3350, 0.2M potassium citrate tribasic monohydrate |