5Z09
ST0452(Y97N)-UTP binding form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.662, 88.417, 179.339 |
| Unit cell angles | 90.00, 121.59, 90.00 |
Refinement procedure
| Resolution | 152.760 - 2.910 |
| R-factor | 0.21176 |
| Rwork | 0.209 |
| R-free | 0.26662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ggo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 153.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.092 | 0.513 |
| Number of reflections | 61139 | 3018 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20%(w/v) PEG3350, 0.2M potassium citrate tribasic monohydrate |
