5Z08

The crystal structure of kinetochore subunits Cenp-H/I/K triple complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-15
Detector	RAYONIX MX-225
Wavelength(s)	0.986
Spacegroup name	P 41
Unit cell lengths	67.847, 67.847, 186.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.975 - 2.199
R-factor	0.1898
Rwork	0.188
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.901
Data reduction software	PHASER
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2463))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.199	2.200
Number of reflections	42600
<I/σ(I)>	29.6
Completeness [%]	100.0
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 8000