5Z05

Crystal structure of signalling protein from buffalo (SPB-40) with an acetone induced conformation of Trp78 at 1.49 A resolution

Replaces:  5WSIReplaces:  4S06

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-02
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	60.457, 66.517, 105.847
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.320 - 1.490
R-factor	0.14856
Rwork	0.147
R-free	0.17641
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5WSI
RMSD bond length	0.038
RMSD bond angle	3.117
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.320	1.530
High resolution limit [Å]	1.490	1.490
Number of reflections	65450
<I/σ(I)>	11.4	2.2
Completeness [%]	99.0	99
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	35mM Tris -HCl pH- 8.0, 25mM Nacl, 20% Acetone.