5YZY
AtVAL1 B3 domain in complex with 13bp-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 87.780, 87.780, 148.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.610 |
| R-factor | 0.21238 |
| Rwork | 0.210 |
| R-free | 0.25674 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.695 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.600 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.096 | |
| Number of reflections | 9243 | |
| <I/σ(I)> | 37.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% (w/v) PEG 5000 MME, 0.1M Tris base/Hydrochloric acid, 0.2M Lithium sulfate |
