5YZL
Crystal structure of human Archease D178A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0688 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.686, 59.620, 133.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.730 - 3.400 |
| Rwork | 0.188 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yz1 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 5767 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.6 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 30% PEG 400, 100mM HEPES/Sodium hydroxide pH 7.5, 200mM Magnesium chloride |
