5YZB

Crystal Structure of Human CRMP-2 with S522D-T509D-T514D-S518D mutations crystallized with GSK3b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-28
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	I 41 2 2
Unit cell lengths	114.340, 114.340, 194.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.458 - 2.800
R-factor	0.205
Rwork	0.202
R-free	0.26610
RMSD bond length	0.010
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.819	3.516
Rmeas	0.835	3.583
Number of reflections	16296
<I/σ(I)>	18
Completeness [%]	99.8	99
Redundancy	26.5	27.1
CC(1/2)	0.989	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	14% PEG 3000, 0.1M tri-sodium citrate pH 5.5